Narishige Pc10 Manual New Jun 2026
This guide provides a detailed overview of the PC-10's features, setup, operation, and optimization techniques based on the standard operating procedures. 1. Introduction to the Narishige PC-10
Adjusts the electromagnetic force applied during the second stage pull. This controls the velocity of the pull, directly influencing the tip size and taper length.
Ideal for intracellular recording electrodes or microinjection needles requiring a long, flexible shank. Configure the toggle switch to .
Disconnect the power. Loosen the terminal screws holding the filament legs. Remove the old coil, insert the new Narishige factory-approved platinum filament, center it visually around a test capillary, and firmly tighten the screws. 7. Troubleshooting Guide Probable Cause Corrective Action Glass does not separate Heater settings are too low.
Set the second heater value slightly lower than the first to clean-snap the thinned glass shank, creating a high-angle, blunt patch tip. 5. Maintenance and Filament Replacement narishige pc10 manual new
Hold the glass capillary using precise clamping screws.
These control the pulling force. For a standard sharp tip, set the magnets to a higher value for the initial pull and a lower value for the final pull. Step 4: Activating the Pull Unlock the safety stopper. Press the start button .
Minimizes output voltage variations to ensure the heater reaches the exact same intensity every time.
The PC-10 is a vertical micropipette puller manufactured by the Japanese company Narishige, a name synonymous with precision in life science tools. Its primary function was to fabricate micropipettes and microelectrodes from glass or quartz capillary tubes by applying controlled heat and mechanical force, typically gravity. The device was renowned for its ability to produce tips ranging from one micron down to a tenth of a micron, making it ideal for creating tools for microinjection and patch clamp electrophysiology. This guide provides a detailed overview of the
Store the pulled pipettes in a dust-free, enclosed pipette storage box. 5. Troubleshooting and Maintenance Probable Cause Corrective Action Heater dial set too high or pull weights are too light.
: Set the "No.2 Heater" level. This stage finalizes the tip diameter and shape. 4. Error Messages & Troubleshooting Common error codes for the PC-10 include: : Slider distance set to 0mm. : Set to 1mm or longer. : First pull completion not detected. : Increase No.1 Heater output. : Slider speed too fast during first pull. : Reduce No.1 Heater output or weight. : Heater contact failure or broken heater. : Tighten fixing screws or replace heater.
The process involves loading the glass capillary into the center of the heating element, closing the acrylic shroud, setting the desired parameters, and initiating the pull. The display will show the elapsed time of the process. The PC-10 can be used in "single pull" mode for a simple taper or "double pull" mode to produce more complex tips.
A clear cover protects the pulling area from external air currents, which can otherwise cause inconsistent cooling and tip deformation. This controls the velocity of the pull, directly
Before powering on the unit, familiarize yourself with the front panel controls and mechanical linkages:
Wipe the vertical sliding rods weekly with a drop of low-viscosity machine oil to guarantee smooth carriage travel. Remove excess oil to avoid dust accumulation.
The Narishige PC-10 is a dual-stage vertical glass micropipette puller widely recognized for its reliability in producing consistent, high-quality microelectrodes and micropipettes. This comprehensive manual and operating guide provides step-by-step instructions for installation, configuration, and advanced parameter tuning to achieve optimal results for various electrophysiology and microinjection applications. 1. System Overview and Technical Specifications
The manufacturer's FAQ pages and distributor specification sheets provide the most current official information for the PC-10.
